Antibacterial activity of cardiotoxin-like basic polypeptide from cobra venom

Peter V Dubovskii; Anastasia A Ignatova; Alexey V Feofanov; Yuri N Utkin; Roman G Efremov

doi:10.1016/j.bmcl.2019.126890

Antibacterial activity of cardiotoxin-like basic polypeptide from cobra venom

Bioorg Med Chem Lett. 2020 Feb 1;30(3):126890. doi: 10.1016/j.bmcl.2019.126890. Epub 2019 Dec 16.

Authors

Peter V Dubovskii¹, Anastasia A Ignatova², Alexey V Feofanov², Yuri N Utkin³, Roman G Efremov⁴

Affiliations

¹ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia. Electronic address: pvdubov@yandex.ru.
² Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia; M.V. Lomonosov Moscow State University, Biological Faculty, ul. Leninsky Gory 1, 119234 Moscow, Russia.
³ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
⁴ Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia; Higher School of Economics, ul. Myasnitskaya 20, 101000 Moscow, Russia; Moscow Institute of Physics and Technology (State University), 9 Institutskiy per., Dolgoprudny, Moscow Region 141700 Russia.

PMID: 31870648
DOI: 10.1016/j.bmcl.2019.126890

Abstract

Antibacterial activity of the three-finger toxins from cobra venom, including cytotoxin 3 from N. kaouthia, cardiotoxin-like basic polypeptide A5 from N. naja (CLBP), and alpha-neurotoxin from N. oxiana venom, was investigated. All toxins failed to influence Gram-negative bacteria. The most pronounced activity against Bacillus subtilis was demonstrated by CLBP. The latter is ascribed to the presence of additional Lys-residues within the membrane-binding motif of this toxin.

Keywords: Antibacterial activity; Bacillus subtilis; Cobra venom; Electric charge distribution; Three-finger toxins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / isolation & purification
Anti-Bacterial Agents / pharmacology
Bacillus subtilis / drug effects
Cardiotoxins / chemistry
Elapid Venoms / metabolism*
Elapidae / metabolism
Escherichia coli / drug effects
Microbial Sensitivity Tests
Peptides / chemistry*
Peptides / isolation & purification
Peptides / pharmacology
Protein Structure, Tertiary
Staphylococcus aureus / drug effects

Substances

Anti-Bacterial Agents
Cardiotoxins
Elapid Venoms
Peptides