Leclercia adecarboxylata From Human Gut Flora Carries mcr-4.3 and bla IMP-4-Bearing Plasmids

Qiaoling Sun; Hanyu Wang; Lingbin Shu; Ning Dong; Fan Yang; Hongwei Zhou; Sheng Chen; Rong Zhang

doi:10.3389/fmicb.2019.02805

Leclercia adecarboxylata From Human Gut Flora Carries mcr-4.3 and bla _IMP-4-Bearing Plasmids

Front Microbiol. 2019 Dec 5:10:2805. doi: 10.3389/fmicb.2019.02805. eCollection 2019.

Authors

Qiaoling Sun¹, Hanyu Wang², Lingbin Shu¹, Ning Dong^{3

4}, Fan Yang⁵, Hongwei Zhou¹, Sheng Chen^{3

4}, Rong Zhang¹

Affiliations

¹ Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.
² University of Conneticut, Mansfield, CT, United States.
³ Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China.
⁴ State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, China.
⁵ Department of Biophysics and Kidney Disease Center, The First Affiliated Hospital, Institute of Neuroscience, National Health Commission and Chinese Academy of Medical Sciences Key Laboratory of Medical Neurobiology, Zhejiang University School of Medicine, Hangzhou, China.

Abstract

A clinical Leclercia adecarboxylata strain harboring the mcr-4.3 and bla _IMP-4 genes was isolated from active rectal screening of carbapenem-resistant Enterobacteriaceae (CRE) in a patient. The isolate was found to harbor seven plasmids, including a 94,635 bp bla _IMP-4-bearing IncN plasmid and a 9,782 bp mcr-4.3-bearing ColE10-type plasmid. The isolate was susceptible to colistin despite carrying the mcr-4.3 gene, suggesting that this MCR-4 variant may not be functional. Carriage of antibiotic resistance genes in human gut L. adecarboxylata strain suggests that close surveillance of resistance strains in the human gut flora should be included as a routine clinical practice to prevent occurrence of infections, especially among immunocompromised patients.

Keywords: Leclercia adecarboxylata; carbapenem resistance; colistin-susceptibility; gut flora; mcr-4.3.