Development of a multi-class method to determine nitroimidazoles, nitrofurans, pharmacologically active dyes and chloramphenicol in aquaculture products by liquid chromatography-tandem mass spectrometry

Dongmei Chen; Jean-Michel Delmas; Dominique Hurtaud-Pessel; Eric Verdon

doi:10.1016/j.foodchem.2019.125924

Development of a multi-class method to determine nitroimidazoles, nitrofurans, pharmacologically active dyes and chloramphenicol in aquaculture products by liquid chromatography-tandem mass spectrometry

Food Chem. 2020 May 1:311:125924. doi: 10.1016/j.foodchem.2019.125924. Epub 2019 Dec 3.

Authors

Dongmei Chen¹, Jean-Michel Delmas², Dominique Hurtaud-Pessel², Eric Verdon³

Affiliations

¹ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues Huazhong Agricultural University, MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products (Wuhan), Huazhong Agricultural University, China; ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Residues and Contaminants Analysis Unit, National Reference Laboratory (NRL) for Veterinary Medicinal Product Residues in Food from Animal Origin and European Union Reference Laboratory (EU-RL) for Authorised and Non-Authorised Antimicrobial Veterinary Medicinal Product Residues and for Banned Pharmacologically Active Dyes, Fougeres, France. Electronic address: chendongmei@mail.hzau.edu.cn.
² ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Residues and Contaminants Analysis Unit, National Reference Laboratory (NRL) for Veterinary Medicinal Product Residues in Food from Animal Origin and European Union Reference Laboratory (EU-RL) for Authorised and Non-Authorised Antimicrobial Veterinary Medicinal Product Residues and for Banned Pharmacologically Active Dyes, Fougeres, France.
³ ANSES, French Agency for Food, Environmental and Occupational Health & Safety, Fougères Laboratory, Residues and Contaminants Analysis Unit, National Reference Laboratory (NRL) for Veterinary Medicinal Product Residues in Food from Animal Origin and European Union Reference Laboratory (EU-RL) for Authorised and Non-Authorised Antimicrobial Veterinary Medicinal Product Residues and for Banned Pharmacologically Active Dyes, Fougeres, France. Electronic address: Eric.VERDON@anses.fr.

PMID: 31865112
DOI: 10.1016/j.foodchem.2019.125924

Abstract

LC-MS/MS method was developed for the efficient identification and quantification of 21 banned substances including various nitroimidazoles, nitrofurans, pharmacologically-active dyes and chloramphenicol, respectively in aquaculture products. The sample preparation was started by acid-treatment with 2-nitrobenzaldehyde (NBA) to liberate matrix-bound residues of nitrofurans. A modified QuEChERS method was optimized for the extraction and clean-up of the target analytes. The metabolites of the four conventional nitrofurans (nitrofurantoin, furazolidone, nitrofurazone and furaltadone) and of three other nitrofurans (nifursol, nifuroxazide, and nitrovin), and an underivatizable nitrofuran (nifurpirinol) were simultaneously detected. Furthermore, 21 banned substances were quantified by LC-MS/MS with ESI using one single injection. To evaluate and validate the performance of the method, the criteria of the Decision (EC) no 2002/657 were applied. Decision limit (CCα) of target analytes ranged 0.067-1.655 μg/kg in aquaculture products. The recovery ranged 77.2%-125.6%, and the relative standard deviations of inter-day analyses (RSD) were less than 25%.

Keywords: Aquaculture products; Chloramphenicol; Dyes; LC-MS/MS; Nitrofurans; Nitroimidazoles; Residues.

Publication types

Evaluation Study

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Aquaculture
Chloramphenicol / analysis*
Chromatography, Liquid / methods*
Coloring Agents / analysis*
Drug Residues / analysis
Fishes / growth & development
Food Contamination / analysis
Furazolidone / analysis
Nitroimidazoles / analysis*
Tandem Mass Spectrometry / methods*

Substances

Anti-Bacterial Agents
Coloring Agents
Nitroimidazoles
Furazolidone
Chloramphenicol