Synthesis and antibacterial activity of solanum torvum mediated silver nanoparticle against Xxanthomonas axonopodis pv.punicae and Ralstonia solanacearum

Gulamnabi L Vanti; Mahantesh Kurjogi; K N Basavesha; Nagappa L Teradal; Segula Masaphy; Vijendra B Nargund

doi:10.1016/j.jbiotec.2019.12.009

Synthesis and antibacterial activity of solanum torvum mediated silver nanoparticle against Xxanthomonas axonopodis pv.punicae and Ralstonia solanacearum

J Biotechnol. 2020 Feb 10:309:20-28. doi: 10.1016/j.jbiotec.2019.12.009. Epub 2019 Dec 18.

Authors

Gulamnabi L Vanti¹, Mahantesh Kurjogi², K N Basavesha², Nagappa L Teradal³, Segula Masaphy⁴, Vijendra B Nargund⁵

Affiliations

¹ Department of Nanotechnology, University of Agricultural Sciences, Dharwad, 580007, India; Department of Mycology and Applied Microbiology, Migal Galilee Research Institute and Tel Hai Academic College, Kiryat Shmona, 11016, Israel. Electronic address: nabisam1@gmail.com.
² Department of Nanotechnology, University of Agricultural Sciences, Dharwad, 580007, India.
³ Department of Chemistry, Ben-Gurion University of the Negev, Beer Sheva, 84102, Israel.
⁴ Department of Mycology and Applied Microbiology, Migal Galilee Research Institute and Tel Hai Academic College, Kiryat Shmona, 11016, Israel.
⁵ Department of Nanotechnology, University of Agricultural Sciences, Dharwad, 580007, India. Electronic address: naragundvb@uasd.in.

PMID: 31863800
DOI: 10.1016/j.jbiotec.2019.12.009

Abstract

To meet the food demand of growing population, agricultural productivity needs to be increased by employing safe strategies without harming ecosystem. Silver nanoparticle (AgNP) using a green approach has become a promising substitute to the synthetic pesticides to overcome pest menace. In this study, AgNPs were synthesized from Solanum torvum fruit extract and their bactericidal property against phyto bacteria was shown. UV-vis spectroscopic observation revealed a surface resonance peak of 440 nm corresponding to the formation of AgNPs. Microscopic and particle-size analyses showed a nearly spherical size, with an average diameter of 27 nm. Surface charge and polydispersity index of the synthesized AgNPs were -11.8 mV and 0.29, respectively. Powder X-ray diffraction, energy-dispersive X-ray and Infrared spectroscopy techniques were used to explore phase formation, composition and possible biological molecules involved in AgNP formation. AgNPs exhibited minimum inhibitory concentrations of 6.25 μg mL^-1 and 12.5 μg mL^-1 against bacterial plant pathogens Xanthomonas axonopodis pv. punicae and Ralstonia solanacearum. In-vitro disk-diffusion assay showed inhibition zones of 11.4 ± 1 mm for R. solanacearum and 18.1 ± 1 mm for X. axonopodis pv. punicae treated with 50 μg mL^-1 AgNPs. The AgNPs generated intracellular reactive oxygen species in the pathogens. DNA damage and DNA replication inhibition studies showed genotoxicity of AgNPs to the bacterial cells. A plant toxicity study demonstrated a nontoxic effect of the synthesized NPs. Overall; the results show that AgNPs can be used as an economically feasible, ecologically safe and effective approach to overcome bacterial diseases.

Keywords: Genotoxicity; Pathogens; Ralstonia solanacearum; Silver nanoparticle; Xanthomonas axonopodis pv.punicae.

MeSH terms

Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects
DNA Damage / drug effects
DNA, Bacterial / analysis
Fruit / chemistry
Green Chemistry Technology
Metal Nanoparticles / chemistry*
Microbial Sensitivity Tests
Microscopy, Electron
Particle Size
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Ralstonia solanacearum / drug effects*
Reactive Oxygen Species / metabolism
Silver / chemistry*
Solanum / chemistry*
X-Ray Diffraction
Xanthomonas axonopodis / drug effects*

Substances

Anti-Bacterial Agents
DNA, Bacterial
Plant Extracts
Reactive Oxygen Species
Silver