In the present study, a novel up-converting phosphor technology-based lateral flow (UPT-LF) assay was developed as a point of care testing method for the rapid and quantitative detection of β-Hydroxybutyrate (BHB) in milk and serum samples. BHB is one of the most important serum molecular markers not only in ketosis but also for other metabolic disorders in bovines. The developed UPT-LF assay has high sensitivity compared with existing kits in the market for BHB quantification. The detection limit of UPT-BHB- LF assay, with a coefficient of variation (CV) < 10% was 0.01 mM, which is lower than the clinical diagnosis cutoff value of serum BHB (1 mM); while 0.1 mM is the standard level of BHB in milk samples. The relatively good stability and reproducibility of UPT-LF assay with CV<10% results for both serum and milk samples at various storage temperatures demonstrates the superiority of this method and therefore it could be used as a point of care method for the quantitative detection of BHB in the biological samples for veterinary applications. The claimed performance of this assay along with a shorter detection time makes it a viable point-of-care method for the quantitative detection of BHB in the biological samples of veterinary applications.
Keywords: Ketosis; Lateral flow assay; Up-converting phosphor; β-Hydroxybutyrate.
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