Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a patient with alcoholic liver cirrhosis of Indian ethnicity

Mohsina Khan; Arun Vaidyanath; Deepika Suresh; Bhumika Vaishnav; Arjun Lal Kakrani; Swapnil Patil; Anjali Shiras

doi:10.1016/j.scr.2019.101678

Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a patient with alcoholic liver cirrhosis of Indian ethnicity

Stem Cell Res. 2020 Jan:42:101678. doi: 10.1016/j.scr.2019.101678. Epub 2019 Dec 16.

Authors

Mohsina Khan¹, Arun Vaidyanath¹, Deepika Suresh¹, Bhumika Vaishnav², Arjun Lal Kakrani², Swapnil Patil², Anjali Shiras³

Affiliations

¹ National Centre for Cell Science (NCCS), Pune, Maharashtra 411007, India.
² Dr. D.Y. Patil Medical College, Dr. D.Y. Patil Vidyapeeth (Deemed University), Pune, Maharashtra 411018, India.
³ National Centre for Cell Science (NCCS), Pune, Maharashtra 411007, India. Electronic address: anjali@nccs.res.in.

PMID: 31862609
DOI: 10.1016/j.scr.2019.101678

Abstract

Three induced pluripotent stem cells (iPSC) clones NCCSi007-A, NCCSi007-B and NCCSi007-C were generated from CD4⁺T cells of a 38 years old male patient suffering from liver cirrhosis- alcoholic and minimal hepatic encephalopathy of Indian origin. The CD4⁺T cells of the patient were reprogrammed using integration free, Sendai viral vector system. Each of the three iPSC clones showed high alkaline phosphatase (ALP) activity, expressed pluripotency markers OCT4, SOX2, NANOG, KLF4, SSEA-4, TRA-1-60, showed normal male karyotype (46, XY) and exhibited multi-lineage differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Clone Cells / metabolism*
Humans
India
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Liver Cirrhosis, Alcoholic / genetics*