Abstract
Staphylococcus epidermidis and a Gram negative bacillus (GNB) were isolated in blood cultures from a 68-year-old male with cancer. The GNB was suspicious for Brucella spp., but was identified using 16S rDNA sequencing as Aureimonas altamirensis. The complexity of the identification is described in this case study.
Keywords:
16S sequencing; Aureimonas; Brucella blood culture; Cancer; MALDI-TOF-MS; Next-generation sequencing; PCR; Whole-genome sequencing.
Copyright © 2019 Elsevier Inc. All rights reserved.
MeSH terms
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Aged
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Alphaproteobacteria / drug effects
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Alphaproteobacteria / isolation & purification*
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Anti-Infective Agents / pharmacology
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Bacterial Infections / diagnosis*
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Bacterial Infections / microbiology
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Blood Culture*
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Brucella
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Genome, Bacterial
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Humans
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Male
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Neoplasms / complications
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Neoplasms / microbiology
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Whole Genome Sequencing
Substances
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Anti-Infective Agents
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RNA, Ribosomal, 16S