Gabor domain optical coherence microscopy combined with laser scanning confocal fluorescence microscopy

Changsik Yoon; Yue Qi; Humberto Mestre; Cristina Canavesi; Olivia J Marola; Andrea Cogliati; Maiken Nedergaard; Richard T Libby; Jannick P Rolland

doi:10.1364/BOE.10.006242

Gabor domain optical coherence microscopy combined with laser scanning confocal fluorescence microscopy

Biomed Opt Express. 2019 Nov 14;10(12):6242-6257. doi: 10.1364/BOE.10.006242. eCollection 2019 Dec 1.

Authors

Affiliations

¹ The Institute of Optics, University of Rochester, Wilmot Building, Rochester, New York 14627, USA.
² Department of Biomedical Engineering, University of Rochester, Robert B. Goergen Hall, Rochester, New York 14627, USA.
³ Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, New York 14642, USA.
⁴ LighTopTech Corp., 150 Lucius Gordon Dr., Ste 201, West Henrietta, New York 14586, USA.
⁵ Flaum Eye Institute, Department of Ophthalmology, University of Rochester Medical Center, Rochester, New York 14642, USA.

Abstract

We report on the development of fluorescence Gabor domain optical coherence microscopy (Fluo GD-OCM), a combination of GD-OCM with laser scanning confocal fluorescence microscopy (LSCFM) for synchronous micro-structural and fluorescence imaging. The dynamic focusing capability of GD-OCM provided the adaptive illumination environment for both modalities without any mechanical movement. Using Fluo GD-OCM, we imaged ex vivo DsRed-expressing cells in the brain of a transgenic mouse, as well as Cy3-labeled ganglion cells and Cy3-labeled astrocytes from a mouse retina. The self-registration of images taken by the two different imaging modalities showed the potential for a correlative study of subjects and double identification of the target.

Abstract

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