Preliminary study on the relationship between aflatoxin-bovine serum albumin adducts in blood and aflatoxin M1 levels in milk of dairy cows

Janine Saltzmann; Ya Xu; Yun Yun Gong; Johanna Lindahl; Susanne Kersten; Sven Dänicke; Michael N Routledge

doi:10.1007/s12550-019-00383-7

Preliminary study on the relationship between aflatoxin-bovine serum albumin adducts in blood and aflatoxin M1 levels in milk of dairy cows

Mycotoxin Res. 2020 May;36(2):207-211. doi: 10.1007/s12550-019-00383-7. Epub 2019 Dec 17.

Authors

Janine Saltzmann¹, Ya Xu², Yun Yun Gong³, Johanna Lindahl^{4

5

6}, Susanne Kersten⁷, Sven Dänicke⁷, Michael N Routledge^{2

8}

Affiliations

¹ Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 37, D-38116, Brunswick, Germany. janine.saltzmann@fli.de.
² School of Medicine, University of Leeds, Leeds, UK.
³ School of Food Science and Nutrition, University of Leeds, Leeds, UK.
⁴ International Livestock Research Institute (ILRI), Nairobi, Kenya.
⁵ Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
⁶ Department of Microbiology and Immunology, Uppsala University, Uppsala, Sweden.
⁷ Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 37, D-38116, Brunswick, Germany.
⁸ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, China.

PMID: 31848876
DOI: 10.1007/s12550-019-00383-7

Abstract

The aflatoxin (AF) albumin adduct is often used as a biomarker for aflatoxin exposure in humans. An ELISA method previously used for aflatoxin serum albumin in human blood was used to analyse bovine serum samples (n = 22) collected from dairy cattle during an aflatoxin mitigation study in Kenya. Albumin adduct data were compared with aflatoxin M₁ (AFM₁) levels in corresponding milk samples from these cows. The concentration ranged from < LOD to 487.9 pg/mL for AFM₁ and < LOD and 96.3 pg/mg for aflatoxin-albumin. This study indicates that aflatoxin-albumin adducts could be used as a measure of chronic aflatoxin exposure in dairy cattle.

Keywords: Aflatoxin M1; Aflatoxin-albumin adduct; Biomarker; Dairy cow; ELISA.

MeSH terms

Aflatoxin M1 / analysis*
Aflatoxins / blood*
Albumins
Animal Feed / analysis
Animals
Cattle
Enzyme-Linked Immunosorbent Assay
Farms
Female
Kenya
Limit of Detection
Milk / chemistry*
Serum Albumin, Bovine / analysis*

Substances

Aflatoxins
Albumins
aflatoxin-albumin adduct
Serum Albumin, Bovine
Aflatoxin M1