Rubber residues present harmful impacts on health and environment, besides wasting valuable and huge amounts of rubber. Biological recycling technique is focused here to minimize this problem. A comparison of the biodegradation effect caused by Bacillus subtilis, Pseudomonas aeruginosa, and Streptomyces sp., separately, on vulcanized SBR-rubber during 4 weeks is reported. The surface and molecular analyses were studied by FTIR-ATR, TGA, DSC, TC and SEM/EDS, in addition to the contact angle and crosslinking tests. B. subtilis, P. aeruginosa, and Streptomyces sp. evoked after 4 weeks a loss in v-SBR crosslinks by 17.15, 10.68 and 43.39% and also in the contact angle with water by 14.10, 12.86 and 15.71%, respectively., if compared to Control samples. FTIR findings indicate that the polymeric chain has been partially consumed causing C-C bonds scission indicating the biodegradation and bio-devulcanization phenomena. The bacterial strains caused a carbon loss by 9.15, 5.97 and 4.55% after one week and 16.09, 16.79 and 18.13% after four weeks for B. subtilis, P. aeruginosa, and Streptomyces sp. mediums, respectively. DSC and EDS results are also promising and highlighting Streptomyces sp. strain as the most effective biodegradative one as an alternative and natural mean of degrading vulcanized rubber residues.