The aim of the study was to clearly determine whether selected modern medical materials and three dimensional printing allow for satisfactory viability of human osteoblasts, which is important from the point of view of the subsequent osseointegration process. Moreover, as implants are produced with various topography, the influence of surface roughness on viability of bone cells was evaluated. To conduct the research, primary human osteoblasts (PromoCell) were used. Cells were seeded on samples of glass-reinforced polyetheretherketone (30% of the filling), Ti6Al4V manufactured with the use of selective laser melting technology and forged Ti6Al4V with appropriately prepared variable surface roughness. To assess the viability of the tested cells the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used. Results showed that all evaluated materials do not exhibit cytotoxic properties. Moreover, on their basis it can be concluded that there is a certain surface topography (designated i.a. as roughness equal to approx. Ra = 0.30 μm), which ensures the highest possible viability of human osteoblasts. On the basis of the received data, it can also be concluded that modern glass-reinforced polyetheretherketone or Ti6Al4V produced by rapid prototyping method allow to manufacture implants that should be effectively used in clinical conditions.
Keywords: biomedical material; cytotoxicity; osteoblasts; sinister laser melting; surface roughness.