In vivo evaluation of arsenic-associated behavioral and biochemical alterations in F0 and F1 mice

Abstract

Groundwater contaminated with arsenic (As) is the biggest threat to public health in Bangladesh. The children of As-exposure parents are also exposing to As through drinking water. The effects of As on the children's health of As-exposure parents are poorly understood. An animal study was taken to evaluate the effects of As on behavioral and biochemical changes in F₁ mice. Swiss albino mice were separated into three groups: a) control, b) As-treated F₀ and c) As-treated F₁. Elevated plus maze and Morris water maze tests were used for evaluating anxiety, spatial memory and learning, respectively. We found that the effect of As on anxiety like behavior, spatial memory and learning impairment in As-treated F₁ mice was significantly higher than that of As-treated F₀ mice and control group. Additionally, we also evaluated the effects of As on biochemical parameters by measuring ALT, AST, ALP, BChE, SOD activities and the level of creatinine in As-induced mice, where we found that all of the blood parameters were significantly changed in F₁ generation. A significant portion of As accumulated in the brain, liver and kidney of F₁ mice than F₀ mice. Histological analysis revealed a significant change in tissue damage related to hepatic and renal dysfunctions that might be associated with As-induced biochemical alterations. In conclusion, arsenic plays an important role for the development of As-associated neurological disorders, hepatic toxicities, and renal dysfunctions in both F₀ and F₁ generations. Notably F₁ mice were much more vulnerable to As-exposure than F₀ mice.

Keywords: Anxiety; Arsenic; Blood indices; F(1) generation; Learning; Spatial memory.