Protective Efficacy of Monoclonal Antibodies Neutralizing Alpha-Hemolysin and Bicomponent Leukocidins in a Rabbit Model of Staphylococcus aureus Necrotizing Pneumonia

Antimicrob Agents Chemother. 2020 Feb 21;64(3):e02220-19. doi: 10.1128/AAC.02220-19. Print 2020 Feb 21.

Abstract

Staphylococcus aureus is a major human pathogen that causes a wide range of infections by producing an arsenal of cytotoxins. We found that passive immunization with either a monoclonal antibody (MAb) neutralizing alpha-hemolysin or a broadly cross-reactive MAb neutralizing Panton-Valentine leukocidin, leukocidin ED, and gamma-hemolysins HlgAB and HlgCB conferred only partial protection, whereas the combination of those two MAbs conferred significant protection in a rabbit model of necrotizing pneumonia caused by the USA300 methicillin-resistant S. aureus epidemic clone.

Keywords: Staphylococcus aureus; acute lung inflammation; necrotizing pneumonia; therapeutic antibodies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / drug therapy
  • Acute Lung Injury / immunology
  • Acute Lung Injury / microbiology
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / therapeutic use*
  • Disease Models, Animal
  • Hemolysin Proteins / immunology*
  • Inflammation / drug therapy
  • Inflammation / immunology
  • Inflammation / microbiology
  • Leukocidins / therapeutic use*
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Methicillin-Resistant Staphylococcus aureus / pathogenicity
  • Pneumonia, Necrotizing / drug therapy*
  • Pneumonia, Necrotizing / immunology*
  • Rabbits
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / pathogenicity

Substances

  • Antibodies, Monoclonal
  • Hemolysin Proteins
  • Leukocidins