Screening and mutagenesis of high-efficient degrading bacteria of linear alkylbenzene sulfonates

Abstract

As a widely used detergent, anionic surfactant linear alkylbenzene sulfonates (LAS) is a common toxic pollutant in wastewater. In this study, Pseudomonas sp. strain H6 was isolated from activated sludge and municipal wastewater, which had good degradation effect on LAS. The results showed that strain H6 could grow with LAS as the sole carbon source. When the concentration of LAS was less than 100 mg/L, strain H6 could degrade more than 80% of the LAS within 24 h. Meanwhile, the growth of strain H6 increased with the increase of LAS concentration, reaching the maximum growth at the presence of 100 mg/L LAS. When the concentration of LAS was over 100 mg/L, strain H6's cell growth and degradation of LAS showed a downward trend due to the strong toxicity of LAS, and the degradation rate of LAS almost tended to zero with 500 mg/L LAS. Further mutagenesis analysis of strain H6 showed that positive mutation occurred under ultraviolet and nitrite mutagenesis with using ampicillin to increase the screening pressure, and the degradation rate of LAS was 44.91% higher than that of original strain.

Keywords: Biodegradation; Linear alkylbenzene sulfonates (LAS); Pseudomonas sp.; Screening; Wastewater.