Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide

Malgorzata Trebacz; Yansong Wang; Leslie Makotta; Lars Henschke; Maja Köhn

doi:10.1021/acs.joc.9b02548

Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide

J Org Chem. 2020 Feb 7;85(3):1712-1717. doi: 10.1021/acs.joc.9b02548. Epub 2019 Dec 31.

Authors

Malgorzata Trebacz^{1

2

3}, Yansong Wang³, Leslie Makotta³, Lars Henschke³, Maja Köhn^{1

2

3}

Affiliations

¹ Faculty of Biology, Institute of Biology III , University of Freiburg , Schänzlestraße 18 , 79104 Freiburg , Germany.
² Signalling Research Centres BIOSS and CIBSS , University of Freiburg , 79104 Freiburg , Germany.
³ European Molecular Biology Laboratory , Genome Biology Unit, Meyerhofstraße 1 , 69117 Heidelberg , Germany.

Abstract

We describe here the development of a photoreleasable version of a protein phosphatase-1 (PP1)-disrupting peptide (PDP-Nal) that triggers protein phosphatase-1 activity. PDP-Nal is a 23 mer that binds to PP1 through several interactions. It was photocaged on a tyrosine residue, which required the exchange of phenylalanine in PDP-Nal to tyrosine in order to disrupt the most important binding interface. This PDP-caged can be light-controlled in live cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Peptides*
Protein Phosphatase 1

Substances

Peptides
Protein Phosphatase 1