Comprehensive fitness evaluation of common carp (Cyprinus carpio L.) after twelve weeks of atrazine exposure

Abstract

Atrazine is frequently detected in surface waters and negatively affect physiological function in aquatic organisms. Even though numerous authors have intensively studied its toxicity, only limited information concerning the long-term fish exposure is available. The aim of this study was evaluation of chronic exposure in common carp. Fish were exposed to a range of atrazine concentrations (0.3 - environmentally relevant concentration; 300; 1000; and 3000 µg/l) for twelve weeks. The potential impact of atrazine exposure was studied using various markers (behaviour; biometrical characteristics; haematological, biochemical and oxidative stress indices and histopathological changes). Most alterations were recorded at the highest concentration (3000 µg/l) which is probably due to a combined effect of both the herbicide exposure and intensive parasite infection development during second week. This group was cancelled after three weeks due to adverse health status, which partly confirmed atrazine immunotoxicity. Chronic exposure resulted in long-term reduction in feed intake followed by a significant decrease in body weight and morphological changes in gill at 1000 µg/l. At the same concentration, significant alterations in haematological (e.g. increase in erythrocyte, leukocyte, lymphocytes and neutrophil counts as well as decrease in monocyte counts) and biochemical (e.g. changes in enzyme activities; increase in glucose; decrease in ammonia) indices were documented, especially during first three weeks. Similar trend, but not so intensive, was observed at 300 µg/l. Moreover, significant changes were observed in various indicators of oxidative stress. These alterations were highly variable with both increasing and decreasing trends depending on dose and analysed tissue. Significant changes, especially in white blood profile, enzyme activities and oxidative stress indices, were proven even at 0.3 µg/l. Obtained results indicate that chronic atrazine exposure of common carp can negatively influence many indicators of health status such as behaviour, immune response, haematological and biochemical profile, oxidative stress indices and organ histopathology.

Keywords: Fish; Haematological and biochemical indices; Histological changes; Oxidative stress; Parasite occurrence; Triazine herbicide.