Objective: To study the PTEN gene expression level and its clinical significance in patients with acute T lymphoblastic leukemia.
Methods: One hundred and twenty-four patients with T-ALL treated in our hospital from January 2014 to May 2018 were selected, and 120 healthy people were selected as control group. The bone marrow of the patients were collected, and mononuclear cells were separated out. PTEN gene expression level was detected by RT-PCR, PTEN protein expression level was detected by Western blot, Kaplan-Meier was used to analyze the survival rate of patients with T-ALL, Cox multivariate regression analyzed was used to analyze the independent influencing factors of patients, and the correlation between PTEN level and clinical characteristics of patients with T-ALL and its prognostic value were analyzed.
Results: The relative level of PTEN gene in patients with T-ALL was 0.19±0.06, which was significantly lower than that in control groups (P<0.05). There was significantly positive correlation of the expression level of PTEN gene with white blood cell count （r=0.993）and peripheral blood immature cells （r=0.996） in patients with T-ALL. There was no correlation between PTEN gene expression level and sex, age, LDH level, Hb level, platelet count in patients with T-ALL. And it was found that expression levels PTEN protein in the middle-risk group, the standard-risk group and the high-risk group were significant lower than those in the control group (P<0.05), while those in the high-risk group were significantly lower than those in other groups (P<0.05). Kaplan-Meier survival analysis showed that OS and DFS in PTEN gene high expression group were higher than those in PTEN low expression group (P<0.05). Cox multivariate regression analysis showed that white blood cell count and PTEN gene were independent influencing factors of OS (P<0.05); platelet count and PTEN gene were independent influencing factors of DFS (P<0.05).
Conclusion: PTEN gene level relates to the prognosis of patients with T-ALL. Patients with better prognosis show a higher PTEN gene level, which provides reference for clinical treatment of patients with T-ALL.
题目: PTEN基因在急性T淋巴细胞白血病患者中的表达水平及临床价值.
目的: 研究急性T淋巴细胞白血病患者PTEN基因表达水平及临床意义.
方法: 选取自2014年1月-2018年5月在本院接受治疗的T-ALL患者124例，同时选取120例健康体检者作为对照组，收集受试者骨髓后分离单个核细胞，通过RT-PCR检测PTEN基因表达水平，采用Western blot检测PTEN蛋白表达水平，Kaplan-Meier法对T-ALL患者进行生存分析，Cox多因素分析患者预后的独立影响因素，并研究PTEN基因水平与T-ALL患者临床特征的相关性及其预后价值.
结果: T-ALL患者的PTEN基因相对水平（0.19±0.06）显著低于健康者（P<0.05）。PTEN表达水平与T-ALL患者的白细胞计数（r=0.993）及外周血幼稚细胞（r=0.996）呈显著相关关系。PTEN基因表达水平与T-ALL患者的性别、年龄、LDH水平、Hb水平及血小板数无相关性。中危组、标危组及高危组患者PTEN蛋白表达水平显著低于对照组（P<0.05）；高危组患者PTEN蛋白表达水平显著低于其它各组（P<0.05）。PTEN基因高表达组的OS及DFS均高于PTEN低表达组（P<0.05）。Cox多因素回归分析表明，白细胞计数及PTEN基因是 OS 独立影响因素（P<0.05）；血小板计数及PTEN基因是DFS独立影响因素（P<0.05）.
结论: PTEN基因水平与T-ALL患者预后相关，预后较好的患者PTEN基因水平较高.