Municipal wastewater effluent influences dissolved organic matter quality and microbial community composition in an urbanized stream

Abstract

Dissolved organic matter (DOM) from wastewater treatment plant (WWTP) effluent poses serious threats to the receiving aqueous ecosystems and their microbial communities. However, the correlation between effluent-derived DOM and microbial community diversity in urbanized rivers is still poorly understood. In this study, the response relationship between the microbial community dynamics and the DOM evolution process in the effluent-dominated Xiaohe River was revealed. The results showed that macromolecular humic acids were the main components of DOM in this river with more carboxylic acid groups and humic-like acid substances found upstream and protein-like substances dominated downstream. The bacterial abundance in the upstream section of Xiaohe River was low, while its community structure was unstable but exhibited good uniformity, and the bacterial diversity in the downstream was rich. The response of bacterial and eukaryotic communities to WWTP effluent was weak, while that of Actinobacteria to WWTP effluent was more prominent. Furthermore, different microbial communities were affected by different compositions and structure of DOM in the effluent of WWTP. The protein-like components in DOM had the most profound impact on the microbial community, followed by polysaccharides and components rich in hydroxyl and amino functional groups. The study grasped the migration and evolution of DOM in rivers with unconventional water recharge, and revealed their diverse effects on microbial community in urbanized rivers.

Keywords: Dissolved organic matter (DOM); Effluent-dominated stream; Microbial community composition.