An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies

J Virol Methods. 2020 Mar:277:113800. doi: 10.1016/j.jviromet.2019.113800. Epub 2019 Dec 16.

Abstract

During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 and 1:300) in a duplicate format, the test was shown to be highly sensitive, specific and repeatable. In comparison to the VNT and a commercial ELISA, the LSDV-IPMA was able to detect the LSDV antibodies earlier in infected, vaccinated and vaccinated/infected animals. The assay is very flexible as it can be easily adapted for the detection of sheeppox or goatpox antibodies and it can be scaled-up to handle medium size sample sets by preparing the IPMA plates in advance. These plates are safe and can be handled in low biosafety level labs.

Keywords: Antibodies; IPMA; LSDV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / immunology
  • Antibodies, Viral / isolation & purification*
  • Cattle
  • Cell Culture Techniques
  • Cell Line
  • Goat Diseases / diagnosis
  • Goat Diseases / immunology
  • Goat Diseases / virology
  • Goats
  • Immunoenzyme Techniques / methods*
  • Lumpy Skin Disease / diagnosis*
  • Lumpy Skin Disease / immunology*
  • Lumpy skin disease virus / immunology*
  • Sensitivity and Specificity
  • Sheep
  • Sheep Diseases / diagnosis
  • Sheep Diseases / immunology
  • Sheep Diseases / virology

Substances

  • Antibodies, Viral