Mathematical model for β1-adrenergic regulation of the mouse ventricular myocyte contraction

Am J Physiol Heart Circ Physiol. 2020 Feb 1;318(2):H264-H282. doi: 10.1152/ajpheart.00492.2019. Epub 2019 Dec 13.

Abstract

The β1-adrenergic regulation of cardiac myocyte contraction plays an important role in regulating heart function. Activation of this system leads to an increased heart rate and stronger myocyte contraction. However, chronic stimulation of the β1-adrenergic signaling system can lead to cardiac hypertrophy and heart failure. To understand the mechanisms of action of β1-adrenoceptors, a mathematical model of cardiac myocyte contraction that includes the β1-adrenergic system was developed and studied. The model was able to simulate major experimental protocols for measurements of steady-state force-calcium relationships, cross-bridge release rate and force development rate, force-velocity relationship, and force redevelopment rate. It also reproduced quite well frequency and isoproterenol dependencies for intracellular Ca2+ concentration ([Ca2+]i) transients, total contraction force, and sarcomere shortening. The mathematical model suggested the mechanisms of increased contraction force and myocyte shortening on stimulation of β1-adrenergic receptors is due to phosphorylation of troponin I and myosin-binding protein C and increased [Ca2+]i transient resulting from activation of the β1-adrenergic signaling system. The model was used to simulate work-loop contractions and estimate the power during the cardiac cycle as well as the effects of 4-aminopyridine and tedisamil on the myocyte contraction. The developed mathematical model can be used further for simulations of contraction of ventricular myocytes from genetically modified mice and myocytes from mice with chronic cardiac diseases.NEW & NOTEWORTHY A new mathematical model of mouse ventricular myocyte contraction that includes the β1-adrenergic system was developed. The model simulated major experimental protocols for myocyte contraction and predicted the effects of 4-aminopyridine and tedisamil on the myocyte contraction. The model also allowed for simulations of work-loop contractions and estimation of the power during the cardiac cycle.

Keywords: 4-aminopyridine; contraction force; myosin-binding protein C; phosphorylation; tedisamil; troponin I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • Calcium Signaling / drug effects
  • Calcium Signaling / physiology
  • Cardiotonic Agents / pharmacology
  • Carrier Proteins / metabolism
  • Computer Simulation
  • Heart Rate / physiology
  • Heart Ventricles* / cytology
  • Heart Ventricles* / drug effects
  • Mice
  • Models, Theoretical
  • Myocardial Contraction / drug effects
  • Myocardial Contraction / physiology*
  • Myocytes, Cardiac / drug effects
  • Myocytes, Cardiac / physiology*
  • Phosphorylation
  • Potassium Channel Blockers / pharmacology
  • Receptors, Adrenergic, beta-1 / drug effects
  • Receptors, Adrenergic, beta-1 / physiology*
  • Sarcomeres / physiology
  • Troponin I / metabolism
  • Troponin I / physiology

Substances

  • Cardiotonic Agents
  • Carrier Proteins
  • Potassium Channel Blockers
  • Receptors, Adrenergic, beta-1
  • Troponin I
  • myosin-binding protein C