Does environmental stress affect cortisol biodistribution in freshwater mussels?

A Ronja D Binder; Michael W Pfaffl; Felix Hiltwein; Juergen Geist; Sebastian Beggel

doi:10.1093/conphys/coz101

Does environmental stress affect cortisol biodistribution in freshwater mussels?

Conserv Physiol. 2019 Dec 8;7(1):coz101. doi: 10.1093/conphys/coz101. eCollection 2019.

Authors

A Ronja D Binder¹, Michael W Pfaffl¹, Felix Hiltwein², Juergen Geist³, Sebastian Beggel³

Affiliations

¹ Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Weihenstephaner Berg 3, Freising-Weihenstephan, D-85354, Germany.
² Independent Scholar.
³ Aquatic Systems Biology Unit, School of Life Sciences Weihenstephan, Technical University of Munich, Mühlenweg 22, Freising-Weihenstephan, D-85354, Germany.

Abstract

As of today, regulation and physiological purpose of steroid hormones in invertebrates such as mussels are not completely understood. Many studies were able to show their presence, but their origin and genesis are not clear. Nevertheless, knowledge about changes in steroid hormone biodistribution in reaction to treatments could improve our understanding of their physiological functions in these species. Cortisol is a corticosteroid, which is frequently used as a stress biomarker in vertebrates, like fish or higher organisms. The aim of the study was to optimize cortisol extraction from various tissues of mussels, to develop a quantitative ELISA test system, and to study changes in biodistribution of cortisol in reaction to negative and positive stimulation treatments. As model organism, we used Anodonta anatina, a widespread freshwater mussel species native to Europe. We quantified cortisol concentrations in hepatopancreas, mantle, gills, gonads and the foot muscle. Tissue-specific reactions to environmental influences, simulated with the chemical stressors copper (II) chloride and sodium chloride, were assessed. During the 24-hours treatment, we additionally observed changes in cortisol regulation in response to feeding activity of the mussels. Besides, we found highly significant variations in the biodistribution of cortisol in different tissues, with a peak in the hepatopancreas. Whole body cortisol did not increase in the treated groups. However, balancing of all measured tissues showed redistribution of more than 10% of total body cortisol from the hepatopancreas to all other tissues during copper (II) chloride stressor treatment, but also when mussels ingested feed, compared to the non-fed control group. No redistribution was observed during sodium chloride treatment. We conclude that there can be a redistribution of cortisol in mussels, depending on external influences.

Keywords: Anodonta anatina; bioindication; bivalves; cortisol ELISA; invertebrates; steroid hormones.