LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis

Luyang Zhang; Yunjian Wang; Ling Zhang; Guohua You; Congyu Li; Bo Meng; Minghe Zhou; Min Zhang

doi:10.1186/s12935-019-1036-2

LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis

Cancer Cell Int. 2019 Dec 2:19:320. doi: 10.1186/s12935-019-1036-2. eCollection 2019.

Authors

Luyang Zhang¹, Yunjian Wang¹, Ling Zhang¹, Guohua You¹, Congyu Li², Bo Meng¹, Minghe Zhou¹, Min Zhang¹

Affiliations

¹ 1Department of Hepatobiliary Surgery, Affiliated of Cancer Hospital of Zhengzhou University, 127 Dongming Road, Zhengzhou, 450008 Henan China.
² 2Department of Ultrasonography, Affiliated of Cancer Hospital of Zhengzhou University, Zhengzhou, 450008 Henan China.

Abstract

Background: Pancreatic cancer (PC) is one of the deadliest cancers about the digestive system. Recent researches have validated that long non-coding RNAs (lncRNAs) play vital roles in various cancers, while the function of LINC01006 in PC is rarely clarified.

Aim of the study: Investigation of the specific role of LINC01006 in PC.

Methods: LINC01006 expression was examined by RT-qPCR. CCK-8, EdU, transwell, wound healing, and western blot assays were carried out to explore the function of LINC01006 in PC. The interaction among LINC01006, miR-2682-5p and HOXB8 was verified by luciferase reporter, RIP and ChIP assays.

Results: The expression of LINC01006 was markedly upregulated in PC tissues and cells. Furthermore, LINC01006 knockdown inhibited PC cell proliferation, invasion and migration, and upregulation of LINC01006 led to the opposite results. Besides, miR-2682-5p expression was downregulated and negatively regulated by LINC01006 in PC. Meanwhile, LINC01006 could bind with miR-2682-5p in PC. Moreover, miR-2682-5p negatively regulated HOXB8 expression and there was a binding site between miR-2682-5p and HOXB8 in PC. Additionally, miR-2682-5p overexpression or HOXB8 knockdown rescued the promotive effects of LINC01006 upregulation on PC cell progression. Similarly, miR-2682-5p inhibition or HOXB8 overexpression countervailed the repressive role of LINC01006 downregulation in PC cell progression. In addition, the transcription factor HOXB8 could activate LINC01006 transcription in PC.

Conclusions: LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis, which may facilitate the treatment for PC.

Keywords: HOXB8; LINC01006; PC; miR-2682-5p.