Relationship between Helicobacter pylori Infection and Plasmacytoid and Myeloid Dendritic Cells in Peripheral Blood and Gastric Mucosa of Children

Abstract

Purpose: To investigate the frequency and activation status of peripheral plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) as well as gastric mucosa DC subset distribution in Helicobacter pylori- (H. pylori-) infected and noninfected children.

Materials and methods: Thirty-six children were studied; twenty-one had H. pylori. The frequencies of circulating pDCs (lineage^-HLA-DR⁺CD123⁺) and mDCs (lineage^-HLA-DR⁺CD11c⁺) and their activation status (CD83, CD86, and HLA-DR expression) were assessed by flow cytometry. Additionally, the densities of CD11c⁺, CD123⁺, CD83⁺, CD86⁺, and LAMP3⁺ cells in the gastric mucosa were determined by immunohistochemistry.

Results: The frequency of circulating CD83⁺ mDCs was higher in H. pylori-infected children than in the noninfected controls. The pDCs demonstrated upregulated HLA-DR surface expression, but no change in CD86 expression. Additionally, the densities of gastric lamina propria CD11c⁺ cells and epithelial pDCs were increased. There was a significant association between frequency of circulating CD83⁺ mDCs and gastric lamina propria mDC infiltration.

Conclusion: This study shows that although H. pylori-infected children had an increased population of mature mDCs bearing CD83 in the peripheral blood, they lack mature CD83⁺ mDCs in the gastric mucosa, which may promote tolerance to local antigens rather than immunity. In addition, this may reduce excessive inflammatory activity as reported for children compared to adults.