Structure and bioactivity of sulfated α-D-mannan from marine bacterium Halomonas halocynthiae KMM 1376T

Maxim S Kokoulin; Alina P Filshtein; Lyudmila A Romanenko; Irina V Chikalovets; Oleg V Chernikov

doi:10.1016/j.carbpol.2019.115556

Structure and bioactivity of sulfated α-D-mannan from marine bacterium Halomonas halocynthiae KMM 1376^T

Carbohydr Polym. 2020 Feb 1:229:115556. doi: 10.1016/j.carbpol.2019.115556. Epub 2019 Nov 4.

Authors

Maxim S Kokoulin¹, Alina P Filshtein², Lyudmila A Romanenko², Irina V Chikalovets³, Oleg V Chernikov²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia; Far Eastern Federal University, 8, Sukhanova str., Vladivostok, 690950, Russia.

PMID: 31826483
DOI: 10.1016/j.carbpol.2019.115556

Abstract

Halomonas halocynthiae KMM 1376^T is a Gram-negative bacterium that has been isolated from gill tissue of the ascidian Halocynthia aurantium. Mild acid hydrolysis of the lipopolysaccharide of H. halocynthiae KMM 1376^T afforded an O-polysaccharide, which was studied by sugar analysis and NMR spectroscopy. The following structure of the O-polysaccharide presented as sulfated α-D-mannan was established: →2)-α-D-Manp3,6S-(1→3)-α-D-Manp2Ac(∼71%)6S-(1→3)-α-D-Manp-(1→. Study of biological activity has shown that sulfated α-D-mannan can specifically reduce the cell viability and colony formation of the human breast adenocarcinoma MDA-MB-231 cells in a concentration-dependent manner. In addition, polysaccharide inhibits epidermal growth factor induced neoplastic cell transformation in mouse epidermal JB6 Cl41 cells.

Keywords: Bacterium; Halomonashalocynthiae; MDA-MB-231; Marine; Neoplastic cell transformation; O-polysaccharide; Sulfated mannan.

MeSH terms

Acetates / chemistry
Animals
Cell Line, Tumor
Cell Survival / drug effects
Cell Transformation, Neoplastic / drug effects
Epidermal Growth Factor / pharmacology
Halomonas / metabolism*
Humans
Hydrolysis
Lipopolysaccharides / chemistry
Mannans / chemistry*
Mannans / pharmacology
Mice
Polysaccharides, Bacterial / chemistry
Polysaccharides, Bacterial / pharmacology
Sulfates / chemistry

Substances

Acetates
Lipopolysaccharides
Mannans
Polysaccharides, Bacterial
Sulfates
Epidermal Growth Factor