GmPGL1, a Thiamine Thiazole Synthase, Is Required for the Biosynthesis of Thiamine in Soybean

Xingxing Feng; Suxin Yang; Kuanqiang Tang; Yaohua Zhang; Jiantian Leng; Jingjing Ma; Quan Wang; Xianzhong Feng

doi:10.3389/fpls.2019.01546

GmPGL1, a Thiamine Thiazole Synthase, Is Required for the Biosynthesis of Thiamine in Soybean

Front Plant Sci. 2019 Nov 22:10:1546. doi: 10.3389/fpls.2019.01546. eCollection 2019.

Authors

Xingxing Feng^{1

2}, Suxin Yang¹, Kuanqiang Tang^{1

2}, Yaohua Zhang¹, Jiantian Leng¹, Jingjing Ma^{1

2}, Quan Wang^{1

2}, Xianzhong Feng¹

Affiliations

¹ Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of eography and Agroecology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Changchun, China.
² College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.

Abstract

Thiamine is an essential cofactor in several enzymatic reactions for all living organisms. Animals cannot synthesize thiamine and depend on their diet. Enhancing the content of thiamine is one of the most important goals of plant breeding to solve the thiamine deficiency associated with the low-thiamin staple crops. In this study, a Glycine max pale green leaf 1 (Gmpgl1) mutant was isolated from the EMS mutagenized population of soybean cultivar, Williams 82. Map-based cloning of the GmPGL1 locus revealed a single nucleotide deletion at the 292th nucleotide residue of the first exon of Glyma.10g251500 gene in Gmpgl1 mutant plant, encoding a thiamine thiazole synthase. Total thiamine contents decreased in both seedlings and seeds of the Gmpgl1 mutant. Exogenous application of thiazole restored the pale green leaf phenotype of the mutant. The deficiency of thiamine in Gmpgl1 mutant led to reduced activities of the pyruvate dehydrogenase (PDH) and pyruvate decarboxylase (PDC), and decreased contents of six amino acids as compared to that in the wild type plants. These results revealed that GmPGL1 played an essential role in thiamine thiazole biosynthesis.

Keywords: GmPGL1; amino acid synthesis; carbohydrate synthesis; soybean; thiamine thiazole synthase.