Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)-An Electrochemical Optimisation Approach

Lisa Crossley; Bukola Attoye; Vincent Vezza; Ewen Blair; Damion K Corrigan; Stuart Hannah

doi:10.3390/bios9040141

Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)-An Electrochemical Optimisation Approach

Biosensors (Basel). 2019 Dec 6;9(4):141. doi: 10.3390/bios9040141.

Authors

Lisa Crossley¹, Bukola Attoye¹, Vincent Vezza¹, Ewen Blair¹, Damion K Corrigan¹, Stuart Hannah¹

Affiliation

¹ Department of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UK.

Abstract

We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences.

Keywords: TP53; biosensor; electrochemistry; field-effect transistor; open-circuit potential.

MeSH terms

Biosensing Techniques*
DNA / chemistry*
Electrochemical Techniques*
Humans
Mutation
Nucleic Acid Hybridization
Transistors, Electronic*
Tumor Suppressor Protein p53 / genetics*

Substances

Tumor Suppressor Protein p53
DNA

Abstract

MeSH terms

Substances

Grants and funding