Preparation of monodisperse porous polymeric ionic liquid microspheres and their application as stationary phases for HPLC

Qiaohong Peng; Yue Wu; Hailin Cong; Youqing Shen; Khalid Mahmood; Bing Yu

doi:10.1016/j.talanta.2019.120462

Preparation of monodisperse porous polymeric ionic liquid microspheres and their application as stationary phases for HPLC

Talanta. 2020 Feb 1:208:120462. doi: 10.1016/j.talanta.2019.120462. Epub 2019 Oct 10.

Authors

Qiaohong Peng¹, Yue Wu¹, Hailin Cong², Youqing Shen², Khalid Mahmood³, Bing Yu⁴

Affiliations

¹ Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China.
² Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China; State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao, 266071, China.
³ Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
⁴ Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China; State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao, 266071, China. Electronic address: yubing198@qdu.edu.cn.

PMID: 31816741
DOI: 10.1016/j.talanta.2019.120462

Abstract

In recent decades, ionic liquid-modified packing materials as stationary phases of high performance liquid chromatography (HPLC) have attracted widespread attention due to their comprehensive properties. In this work, monodisperse porous polymeric ionic liquid microspheres were directly prepared using a seeded emulsion polymerization method and applied as stationary phase of HPLC. The effects of porogen, monomer and crosslinker on the properties of porous P(IL-DVB) microspheres were investigated in detail. Porous P(IL-DVB) microspheres with good stability, monodispersity and relatively high specific surface area were selected as stationary phase to successfully separate benzene and its homologues, and nucleosides and alkaloids under reversed phase chromatography (RPLC) mode and hydrophilic interaction chromatography (HILIC) mode, respectively.

Keywords: High performance liquid chromatography; Mix-mode separation; Polymeric ionic liquid; Porous microspheres; Seeded emulsion polymerization.