Bioactive compounds demonstrating antioxidant activity were analyzed in berries and leaves of nine cultivars of sea buckthorn (Hippophaë rhamnoides L.) of various ripening times. Total polyphenols were ranging between 0.70-3.62 g GAE.kg-1 (berries) and 1.88-3.72 g GAE.kg-1 (leaves). Leaves were significantly richer source of total flavonoids (14.40-49.44 mg RE.kg-1) in comparison with berries (0.55-4.11 mg RE.kg-1). Phenolic compounds, carotenoids and vitamins were determined using high-performance liquid chromatography with a diode array detection. The content of vitamin C was 0.98-3.65 g.kg-1 in berries and 22.81-46.32 g.kg-1 in leaves, vitamin E content was 6.98-29.91 g.kg-1 in berries and 71.54-153.99 g.kg-1 in leaves. Distribution of individual phenolic compounds varied, their total content in berries was considerably lower (76.1-205.2 mg.kg-1) than in leaves (1477.7-8709.0 mg.kg-1). Regarding antioxidant activity, Raisa and Slovan (berries) and Bojan and Maslicnaja (leaves) were evaluated as the best cultivars.
Keywords: Antioxidant activity; Carotenoids; Catechin (PubChem CID: 9064); Correlation; DPPH (PubChem CID: 2735032); Ellagic acid (PubChem CID: 5281855); HPLC-DAD; Lutein (PubChem CID: 5281243); Lycopene (PubChem CID: 446925); Neochlorogenic acid (PubChem CID: 5280633); Phenolic compounds; Resveratrol (PubChem CID: 445154); Sea buckthorn; Trolox (PubChem CID: 40634); Vitamins; Zeaxanthin (PubChem CID: 5280899); β-carotene (PubChem CID: 5280489).
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