Exploring insertions and deletions (indels) of MSRB3 gene and their association with growth traits in four Chinese indigenous cattle breeds

Mingli Wu; Shipeng Li; Guoliang Zhang; Yingzhi Fan; Yuan Gao; Yongzhen Huang; Xianyong Lan; Chuzhao Lei; Yun Ma; Ruihua Dang

doi:10.5194/aab-62-465-2019

Exploring insertions and deletions (indels) of MSRB3 gene and their association with growth traits in four Chinese indigenous cattle breeds

Arch Anim Breed. 2019 Jul 25;62(2):465-475. doi: 10.5194/aab-62-465-2019. eCollection 2019.

Authors

Mingli Wu¹, Shipeng Li¹, Guoliang Zhang², Yingzhi Fan¹, Yuan Gao¹, Yongzhen Huang¹, Xianyong Lan¹, Chuzhao Lei¹, Yun Ma^{3

4}, Ruihua Dang¹

Affiliations

¹ Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
² Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, Jilin, 136100, P. R. China.
³ College of Life Sciences, Xinyang Normal University, Xinyang, Henan, 464000, P. R. China.
⁴ School of Agriculture, Ningxia University, Yinchuan, Ningxia, 750021, P. R. China.

Abstract

Methionine sulfoxide reductase B3 (MSRB3) is instrumental in ossification and fat deposition, which regulate the growth and development of cattle directly. The purpose of this study was aimed to explore insertions and deletions (indels) in MSRB3 gene and investigate their association with growth traits in four indigenous cattle breeds (Luxi cattle, Qinchuan cattle, Nanyang cattle, and Jiaxian Red cattle). Four indels were identified by sequencing with DNA pool. Association analysis showed that three of them were associated with growth traits ( $P < 0.05$ ). For P1, the DD (deletion and deletion) genotype was significantly associated with body length of Nanyang cattle; for P6, II (insertion and insertion) and/or DD genotypes were significantly associated with enhanced growth traits of Qinchuan cattle; for P7, II genotype was significantly associated with hucklebone width of Luxi cattle. Our results demonstrated that the polymorphisms in bovine MSRB3 gene were significantly associated with growth traits, which could be candidate loci for marker-assisted selection (MAS) in cattle breeding.