Combination of mineral trioxide aggregate and propolis promotes odontoblastic differentiation of human dental pulp stem cells through ERK signaling pathway

Jae-Hwan Kim; Soo-Yung Kim; Su-Mi Woo; Ha-Na Jeong; Ji-Yeon Jung; Seon-Mi Kim; Hae-Soon Lim

doi:10.1007/s10068-019-00609-5

Combination of mineral trioxide aggregate and propolis promotes odontoblastic differentiation of human dental pulp stem cells through ERK signaling pathway

Food Sci Biotechnol. 2019 May 21;28(6):1801-1809. doi: 10.1007/s10068-019-00609-5. eCollection 2019 Dec.

Authors

Jae-Hwan Kim¹, Soo-Yung Kim¹, Su-Mi Woo², Ha-Na Jeong³, Ji-Yeon Jung², Seon-Mi Kim¹, Hae-Soon Lim^{3

4}

Affiliations

¹ 1Department of Pediatric Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Yongbong-ro 33, Buk-gu, Gwangju, Republic of Korea.
² 2Department of Oral Physiology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Yongbong-ro 33, Buk-gu, Gwangju, Republic of Korea.
³ 3Department of Dentistry, Chonnam National University Hwasun Hospital, Seoyang-ro 322, Ilsim-ri, Hwasun-eup, Hwasun-gun, Jeollanam-do Republic of Korea.
⁴ 4Department of Dental Education, School of Dentistry, Dental Science Research Institute, Chonnam National University, Yongbong-ro 33, Buk-gu, Gwangju, 61186 Republic of Korea.

Abstract

The aim of this study is to investigate combined effects of mineral trioxide aggregate (MTA) and propolis on odontoblastic differentiation of human dental pulp stem cells (DPSCs) and to find a signaling pathway involved. Combination of MTA and propolis significantly up-regulated the expression of DSPP and DMP1, and facilitated a mineral nodule formation (p < 0.05). Treatments with MTA, propolis or combined increased the phosphorylation of extracellular signal-regulated kinases (ERK), one of mitogen-activated protein kinases signaling cascades during odontogenic differentiation of DPSCs (p < 0.05), and U0126, an inhibitor of ERK, decreased calcium deposits (p < 0.05). Combination of MTA and propolis promotes odontogenic differentiation and mineralization of DPSCs through ERK pathway.

Keywords: ERK signaling; Human dental pulp cells; Mineral trioxide aggregate; Odontoblastic differentiation; Propolis.