Optimization of process parameters for improved production of biomass protein from Aspergillus niger using banana peel as a substrate

Md Mostafa Kamal; Md Rahmat Ali; Mohammad Rezaul Islam Shishir; Md Saifullah; Md Raihanul Haque; Shakti Chandra Mondal

doi:10.1007/s10068-019-00636-2

Optimization of process parameters for improved production of biomass protein from Aspergillus niger using banana peel as a substrate

Food Sci Biotechnol. 2019 Jun 7;28(6):1693-1702. doi: 10.1007/s10068-019-00636-2. eCollection 2019 Dec.

Authors

Md Mostafa Kamal¹, Md Rahmat Ali¹, Mohammad Rezaul Islam Shishir², Md Saifullah³, Md Raihanul Haque⁴, Shakti Chandra Mondal¹

Affiliations

¹ 1Department of Food Processing and Preservation, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200 Bangladesh.
² 2Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310058 China.
³ 3School of Environmental and Life Sciences, Faculty of Science and Information Technology, University of Newcastle, Central Coast, Ourimbah, NSW 2258 Australia.
⁴ 4Department of Food Engineering and Technology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200 Bangladesh.

Abstract

This study was aimed to optimize the process variables for improved production of biomass protein using Aspergillus niger from banana fruit peel by the use of response surface methodology. A five-level-four factors central composite rotatable design was applied to elucidate the influence of process variables viz. temperature (20-40 °C), pH (4-8), substrate concentration (5-25%), and fermentation period (1-5 days) on biomass and protein content. The second-order polynomial models were established, which effectively explicated the variation in experimental data and significantly epitomized the appreciable correlation between independent variables and responses. After numerical optimization, the predicted optimum conditions (temperature of 31.02 °C, pH of 6.19, substrate concentration of 19.92%, and the fermentation period of 4 days) were obtained with biomass of 24.69 g/L and protein of 61.23%, which were verified through confirmatory experiments.

Keywords: Aspergillus niger; Banana peel; Biomass; Optimization; Protein; Response surface methodology.