Chronic kidney disease (CKD) is a persistent kidney structural and functional disorder. Quercetin is one of active extracted flavonoids and has protective effects. Therefore, we proposed to survey the effect of Quercetin on CKD. HK-2 cells were preprocessed by Quercetin and then irritated with lipopolysaccharide (LPS). CCK-8 assay and flow cytometry were utilized to test viability and apoptosis. ELISA assay was utilized to estimate the IL-6 and TNF-α secretion. Western blot was performed to examine the expression of apoptosis and inflammation-associated mediators. After that HK-2 cells were transfected by miR-124 inhibitor. And the above-mentioned parameters were reassessed. LPS stimulated apoptosis and declined viability in HK-2 cells. Additionally, LPS stimulated inflammatory responses. Meanwhile, Quercetin attenuated LPS-stimulated apoptosis, production of IL-6, and TNF-α in experimental cells. Subsequently, MyD88 and miR-124 expression was elevated by LPS and alleviated by Quercetin. Finally, Quercetin exerted its protective function through NF-κB pathway via up-regulating miR-124. Our data demonstrated that Quercetin reduced apoptosis and inflammation stimulated by LPS in HK-2 cells. Moreover, Quercetin alleviated LPS-stimulated injury by up-regulating miR-124.
Keywords: Quercetin; apoptosis; chronic kidney disease; inflammatory responses; miR-124.
© 2019 International Union of Biochemistry and Molecular Biology.