Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4

Muhammad Asif Nawaz; Sidra Pervez; Muhsin Jamal; Tour Jan; Wali Khan; Abdur Rauf; Afsheen Aman; Shah Ali Ul Qader

doi:10.1016/j.btre.2019.e00400

Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4

Biotechnol Rep (Amst). 2019 Nov 12:24:e00400. doi: 10.1016/j.btre.2019.e00400. eCollection 2019 Dec.

Authors

Muhammad Asif Nawaz¹, Sidra Pervez², Muhsin Jamal³, Tour Jan⁴, Wali Khan⁵, Abdur Rauf⁶, Afsheen Aman⁷, Shah Ali Ul Qader⁸

Affiliations

¹ Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan.
² Department of Microbiology, Hazara University, Mansehra, KPK, Pakistan.
³ Department of Microbiology, Abdul Wali Khan University, Mardan, Pakistan.
⁴ Department of Botany, University of Malakand, Chakdara, Khyberpakhtunkhwa, Pakistan.
⁵ Department of Zoology, University of Malakand, Chakdara, Khyberpakhtunkhwa, Pakistan.
⁶ Department of Chemistry, University of Swabi, Khyberpakhtunkhwa, Pakistan.
⁷ The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, 75270, Pakistan.
⁸ Department of Biochemistry, University of Karachi, Karachi, 75270, Pakistan.

Abstract

Maltase is an economically valuable enzyme that is used to catalyze the hydrolytic process of maltose and yields d-glucose as a product. In this study, the catalytic behavior of maltase was optimized under various physicochemical condition. Results indicated that bacterial maltase exhibited maximum catalytic activity at 45 °C and pH-6.5 after 5.0 min. It presented greater stability within 0.1 M K₂HPO₄ buffer having pH-6.5 and showed 100 % activity even after 1.0 h. It retained 83.6 % and 45.0 % activity at 40 °C after 1.0 and 3.0 h, respectively. The enzyme retained 90.0 % activity at -20 °C even after 60 days. The molecular weight of enzyme was deduced to be 157.2 kDa as calculated using polyacrylamide gel electrophoresis (PAGE) and zymography. It was concluded that the characterized maltase has notable stability profile with reference to temperature, pH and other reaction conditions which anticipates its utilization in various starch and maltose hydrolyzing processes for the synthesis of glucose.

Keywords: Bacillus licheniformis; Catalytic performance; Maltase; Maltose; Stability.