Molecular Modeling of the Interaction Between Stem Cell Peptide and Immune Receptor in Plants

Methods Mol Biol. 2020:2094:67-77. doi: 10.1007/978-1-0716-0183-9_8.

Abstract

Molecular docking enables comprehensive exploration of interactions between chemical moieties and proteins. Modeling and docking approaches are useful to determine the three-dimensional (3D) structure of experimentally uncrystallized proteins and subsequently their interactions with various inhibitors and activators or peptides. Here, we describe a protocol for carrying out molecular modeling and docking of stem cell peptide CLV3p on plant innate immune receptor FLS2.

Keywords: CLV3p; Docking; FLS2; Modeling; Protein peptide interaction; Structure prediction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / genetics
  • Databases, Genetic
  • Flagellin / chemistry
  • Flagellin / genetics
  • Models, Molecular
  • Molecular Docking Simulation / instrumentation
  • Molecular Docking Simulation / methods*
  • Mutation
  • Peptides / chemistry*
  • Protein Binding
  • Protein Kinases / chemistry*
  • Protein Kinases / genetics
  • Sequence Alignment
  • Software

Substances

  • AT2G27250 protein, Arabidopsis
  • Arabidopsis Proteins
  • Peptides
  • Flagellin
  • Protein Kinases
  • FLS2 protein, Arabidopsis