Abstract
Molecular docking enables comprehensive exploration of interactions between chemical moieties and proteins. Modeling and docking approaches are useful to determine the three-dimensional (3D) structure of experimentally uncrystallized proteins and subsequently their interactions with various inhibitors and activators or peptides. Here, we describe a protocol for carrying out molecular modeling and docking of stem cell peptide CLV3p on plant innate immune receptor FLS2.
Keywords:
CLV3p; Docking; FLS2; Modeling; Protein peptide interaction; Structure prediction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis Proteins / chemistry*
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Arabidopsis Proteins / genetics
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Databases, Genetic
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Flagellin / chemistry
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Flagellin / genetics
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Models, Molecular
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Molecular Docking Simulation / instrumentation
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Molecular Docking Simulation / methods*
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Mutation
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Peptides / chemistry*
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Protein Binding
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Protein Kinases / chemistry*
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Protein Kinases / genetics
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Sequence Alignment
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Software
Substances
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AT2G27250 protein, Arabidopsis
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Arabidopsis Proteins
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Peptides
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Flagellin
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Protein Kinases
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FLS2 protein, Arabidopsis