MAIT cells can recognize and respond to several pathogens in a distinct manner. However, many studies are focused on activation by bacteria and by viruses, while reports about molds and MAIT cells are rare. Here, we describe a method to investigate MAIT cell interaction with filamentous fungi from Aspergillus and Mucorales species. This includes preparation of fungal conidia, cell isolation, and analysis by flow cytometry.
Keywords: Activation; Antigen-presenting cell; Aspergillus; Flow cytometry; MAIT cell; Mold; Mucorales.