Fabrication of ultra-sensitive photoelectrochemical aptamer biosensor: Based on semiconductor/DNA interfacial multifunctional reconciliation via 2D-C3N4

Yang Li; Yuyu Bu; Fengqiu Jiang; Xianying Dai; Jin-Ping Ao

doi:10.1016/j.bios.2019.111903

Fabrication of ultra-sensitive photoelectrochemical aptamer biosensor: Based on semiconductor/DNA interfacial multifunctional reconciliation via 2D-C₃N₄

Biosens Bioelectron. 2020 Feb 15:150:111903. doi: 10.1016/j.bios.2019.111903. Epub 2019 Nov 21.

Authors

Yang Li¹, Yuyu Bu², Fengqiu Jiang¹, Xianying Dai³, Jin-Ping Ao¹

Affiliations

¹ Key Laboratory of Wide Band-Gap Semiconductor Materials and Devices, School of Microelectronics, Xidian University, Xi'an, 710071, China.
² Key Laboratory of Wide Band-Gap Semiconductor Materials and Devices, School of Microelectronics, Xidian University, Xi'an, 710071, China. Electronic address: yybu@xidian.edu.cn.
³ Key Laboratory of Wide Band-Gap Semiconductor Materials and Devices, School of Microelectronics, Xidian University, Xi'an, 710071, China. Electronic address: xydai@xidian.edu.cn.

PMID: 31791875
DOI: 10.1016/j.bios.2019.111903

Abstract

In this work, we fabricate a novel bismuth vanadate/two dimensional-carbon nitride/deoxyribonucleic acid (BiVO₄/2D-C₃N₄/DNA) aptamer photoelectrochemical (PEC) sensor, and this sensor provides a record detection sensitivity area (5 × 10^-7 μg/L - 10 μg/L) for Microcystin-LR (MC-LR). Meanwhile, except for MC-LR detection, this sensor presents highly sensitivity for tumor marker, heavy metal ion, antibiotic also by changing the DNA aptamer. Photo charge dynamic and theory calculation results reveal that 2D-C₃N₄ is a key material for multifunctional interface reconciliation of this PEC aptamer sensor. Firstly, it can serve as photogenerated hole oriented-transfer medium from the BiVO₄ photoanode to the detective target; In addition, 2D-C₃N₄ with large area of π electron cloud can fix the DNA aptamer parallelly by π-π bonding with the nucleic acid in the DNA aptamer to shorten the hole transfer distance from the semiconductor to target. So that, a record MC-LR detection sensitivity has been achieved by the 2D-C₃N₄ modified BiVO₄/DNA aptamer sensor.

Keywords: 2D-C(3)N(4) interlayer; Charge transfer; PEC aptamer biosensor; Semiconductor/DNA interface reconciliation; Ultrahigh sensitivity.

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques / methods*
Bismuth / chemistry*
Electrochemical Techniques / methods
Marine Toxins
Microcystins / analysis*
Models, Molecular
Nitriles / chemistry*
Semiconductors
Vanadates / chemistry*
Water / analysis

Substances

Aptamers, Nucleotide
Marine Toxins
Microcystins
Nitriles
Water
bismuth vanadium tetraoxide
Vanadates
cyanogen
cyanoginosin LR
Bismuth