Synergistic hepatoprotective effects of CGplus on CCl4-induced acute injury

Abstract

Ethnopharmacological relevance: Traditional Chinese/Korean medicine suggests "blood stasis ()", "stagnation of vital energy ()" and "dampness and Phlegm ()" as the main etiologies of liver disorders, and multiherbal formulas are generally believed to exert synergistic action.

Aim of the study: The present study aimed to investigate the synergistic hepatoprotective effects of CG^plus (a mixture of Salviae miltiorrhiza, Artemisia iwayomogi and Ammomum xanthioides) compared to those of the individual herbs.

Methods and materials: A total of fifty-six male Balb/C mice were randomly divided into eight groups and were administered water (normal and CCl₄ groups), 100 mg/kg S. miltiorrhiza, A. iwayomogi, or A. xanthioides, 50 or 100 mg/kg CG^Plus or dimethyl dimethoxybiphenyl dicarboxylate (DDB) as a positive control for 4 consecutive days. After a single CCl₄ injection (i.p., 10 mL/kg of 0.2% CCl₄ in olive oil), blood and liver tissues were collected after 18 h of fasting for serum biochemistry, histopathological examination and molecular analyses.

Results: CCl₄ injection induced drastic hepatic injury characterized by a more than 30-fold increase in the release of AST and ALT into the serum. These alterations were significantly attenuated by pretreatment with each of the three herbs, while the effects of the individual herbs were synergistically augmented by CG^Plus pretreatment. The synergistic hepatoprotective actions of CG^Plus were demonstrated consistently by analyses of oxidative stress (oxidative stressors, oxidation products and antioxidant enzymes), pro-/anti-inflammatory cytokines (TNF-ɑ, IL-1β, IL-6, IL-10), and apoptosis (caspase-3, p53 and BAX) and histopathology.

Conclusions: These data suggest that CG^Plus exerts its hepatoprotective effects in a synergistic manner, and further studies are required for clinical application using other chronic models.

Keywords: Acute liver injury; Amomum xanthioides; Artemisia iwayomogi; CG(Plus); Herbal medicine; Salvia miltiorrhiza.