Toxicity assessment of p-choroaniline on Platymonas subcordiformis and its biodegradation

Abstract

The use of p-chloroaniline (PCA) in various aspects leads to its existence and accumulation in the environment. Relevant researches showed that PCA was a prime toxic pollutant that had imposed a serious risk to public health and the environment. This paper investigated the toxicity effects of PCA on Platymonas subcordiformis (P. subcordiformis) and the biodegradation of PCA by the marine microalga. In the toxicity experiments, the EC₅₀ of PCA on P. subcordiformis at 24 h, 48 h, 72 h and 96 h was 41.42, 24.04, 17.15 and 13.05 mg L^-1, respectively. The pigment parameters including chlorophyll a, chlorophyll b, carotenoids, photosynthetic O₂ release rate, respiration O₂ consumption rate and the chlorophyll fluorescence parameters including Fv/Fm, ETR and qP decreased greatly while antioxidant enzyme activities (SOD, CAT) and the chlorophyll fluorescence parameter NPQ increased when P. subcordiformis exposed to PCA compared with the control group. Fv/Fm would be a suitable indicator for assessing the toxicity of PCA in marine environment based on the analysis of Pearson's correlation coefficient and Integrated Biomarker Response (IBR). The degradation assay in P. subcordiformis indicated that the green marine microalga had the ability to remove and degrade PCA, and the order of removal and degradation proportion of PCA was 2 mg L^-1 > 5 mg L^-1>10 mg L^-1. The maximum removal and biodegradation percentage was 54% and 34%, respectively.

Keywords: Biodegradation; P-chloroaniline (PCA); Platymonas subcordiformis; Removal; Toxicity assessment.