Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model

Ramachandran Chelliah; Kandasamy Saravanakumar; Eric Banan-Mwine Daliri; Joong-Hark Kim; Jung-Kun Lee; Hyeon-Yeong Jo; Se-Hun Kim; Sudha Rani Ramakrishnan; Inamul Hasan Madar; Shuai Wei; Momna Rubab; Kaliyan Barathikannan; Fred Kwame Ofosu; Hwang Subin; Park Eun-Ji; Jung Da Yeong; Fazle Elahi; Myeong-Hyeon Wang; Jong Hwan Park; Juhee Ahn; Dong-Hwan Kim; Sung Jin Park; Deog-Hwan Oh

doi:10.1016/j.ijbiomac.2019.10.196

Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model

Int J Biol Macromol. 2020 Jan 15:143:555-572. doi: 10.1016/j.ijbiomac.2019.10.196. Epub 2019 Nov 27.

Authors

Ramachandran Chelliah¹, Kandasamy Saravanakumar², Eric Banan-Mwine Daliri¹, Joong-Hark Kim³, Jung-Kun Lee³, Hyeon-Yeong Jo¹, Se-Hun Kim¹, Sudha Rani Ramakrishnan⁴, Inamul Hasan Madar⁵, Shuai Wei⁶, Momna Rubab¹, Kaliyan Barathikannan¹, Fred Kwame Ofosu¹, Hwang Subin¹, Park Eun-Ji¹, Jung Da Yeong¹, Fazle Elahi¹, Myeong-Hyeon Wang², Jong Hwan Park⁷, Juhee Ahn², Dong-Hwan Kim⁸, Sung Jin Park¹, Deog-Hwan Oh⁹

Affiliations

¹ Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea.
² Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea.
³ Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea; Erom, Co., Ltd, Chuncheon, Gangwon-do 24427, South Korea.
⁴ School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
⁵ Department of Biochemistry, School of Life Science, Bharathidasan University, Thiruchirappalli, Tamilnadu, India.
⁶ Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea; Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
⁷ Laboratory Animal Medicine, Chonnam National University, Gwangju, South Korea.
⁸ Kangwon Institute of Inclusive Technology, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea.
⁹ Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea. Electronic address: deoghwa@kangwon.ac.kr.

PMID: 31785295
DOI: 10.1016/j.ijbiomac.2019.10.196

Abstract

Human-milk-based probiotics play a major role in the early colonization and protection of infants against gastrointestinal infection. We investigated potential probiotics in human milk. Among 41 Lactic acid bacteria (LAB) strains, four strains showed high antimicrobial activity against Escherichia coli 0157:H7, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 14576, Staphylococcus aureus ATCC 19095, and Helicobacter pylori. The selected LAB strains were tested in simulated gastrointestinal conditions for their survival. Four LAB strains showed high resistance to pepsin (82%-99%), bile with pancreatine stability (96%-100%), and low pH (80%-94%). They showed moderate cell surface hydrophobicity (22%-46%), auto-aggregation abilities (12%-34%), and 70%-80% co-aggregation abilities against L. monocytogenes ATCC 15313, S. aureus ATCC 19095, B. cereus ATCC 14576, and E. coli 0157:H7. All four selected isolates were resistant to gentamicin, imipenem, novobiocin, tetracycline, clindamycin, meropenem, ampicillin, and penicillin. The results show that Pediococcus acidilatici is likely an efficient probiotic strain to produce < 3 Kda pediocin-based antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography mass spectrometry and HPLC with the corresponding sequences from class 2 bacteriocin, and based on the molecular docking, the mode of action of pediocin was determined on LipoX complex, further the ¹³C nuclear magnetic resonance structural analysis, which confirmed the antimicrobial peptide as pediocin.

Keywords: (13)C NMR; Antimicrobial peptide (pediocin); LCMS; Pediococcus acidilatici; Probiotic.

MeSH terms

Animals
Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / pharmacology
Bacterial Infections* / drug therapy
Bacterial Infections* / microbiology
Caenorhabditis elegans / microbiology*
Pediocins* / chemistry
Pediocins* / pharmacology
Pediococcus acidilactici / chemistry*
Probiotics* / chemistry
Probiotics* / pharmacology

Substances

Anti-Bacterial Agents
Pediocins