This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices.
Keywords: Contaminants; Enzyme; Hormones; Limit of detection; Sensitivity; Water.