Cudraxanthone D Regulates Epithelial-Mesenchymal Transition by Autophagy Inhibition in Oral Squamous Cell Carcinoma Cell Lines

Su-Bin Yu; Hae-Mi Kang; Dan-Bi Park; Bong-Soo Park; In-Ryoung Kim

doi:10.1155/2019/5213028

Cudraxanthone D Regulates Epithelial-Mesenchymal Transition by Autophagy Inhibition in Oral Squamous Cell Carcinoma Cell Lines

Evid Based Complement Alternat Med. 2019 Oct 31:2019:5213028. doi: 10.1155/2019/5213028. eCollection 2019.

Authors

Su-Bin Yu¹, Hae-Mi Kang^{1

2}, Dan-Bi Park^{1

2}, Bong-Soo Park^{1

2

3}, In-Ryoung Kim^{1

2

3}

Affiliations

¹ Department of Oral Anatomy, School of Dentistry, Pusan National University, 49 Busandaehak-ro, Mulguem-eup, Yangsan-si, Gyeongsangnam-do 50612, Republic of Korea.
² BK21 PLUS Project, School of Dentistry, Pusan National University, 49 Busandaehak-ro, Mulguem-eup, Yangsan-si, Gyeongsangnam-do 50612, Republic of Korea.
³ Dental and Life Science Institute, Pusan National University, 49 Busandaehak-ro, Mulguem-eup, Yangsan-si, Gyeongsangnam-do 50612, Republic of Korea.

Abstract

Cudraxanthone D (CD), derived from the root bark of Cudrania tricuspidata, is a natural xanthone compound. However, the biological activity of CD in terms of human metabolism has been barely reported to date. Autophagy is known as a self-degradation process related to cancer cell viability and metastasis. Herein, we investigated the effects of CD on human oral squamous cell carcinoma (OSCC) metastatic related cell phenotype. We confirmed that CD effectively decreased proliferation and viability in a time- and dose-dependent manner in human OSCC cells. In addition, OSCC cell migration, invasion, and EMT were inhibited by CD. To further determine the underlying mechanism of CD's inhibition of cell metastatic potential, we established the relationship between EMT and autophagy in OSCC cells. Thus, our findings indicated that CD inhibited the potential metastatic abilities of OSCC cells by attenuating autophagy.