Frequency of urinary tract infection and antibiotic sensitivity of uropathogens in patients with diabetes

Kaleem Ullah Zubair; Abdul Haleem Shah; Asher Fawwad; Rubina Sabir; Anum Butt

doi:10.12669/pjms.35.6.115

Frequency of urinary tract infection and antibiotic sensitivity of uropathogens in patients with diabetes

Pak J Med Sci. 2019 Nov-Dec;35(6):1664-1668. doi: 10.12669/pjms.35.6.115.

Authors

Kaleem Ullah Zubair¹, Abdul Haleem Shah², Asher Fawwad³, Rubina Sabir⁴, Anum Butt⁵

Affiliations

¹ Kaleem Ullah Zubair, M.Phil Scholar, Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan.
² Abdul Haleem Shah, Dean, Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan.
³ Asher Fawwad, Ph.D. Chairman and Professor of Biochemistry, Department of Biochemistry, Baqai Medical University, Research Director, Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Karachi, Pakistan.
⁴ Rubina Sabir, M.Sc, Laboratory Manager, Department of Clinical and Research Laboratory, Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Karachi, Pakistan.
⁵ Anum Butt, M.Phil, Research Officer, Research Department, Baqai Institute of Diabetology and Endocrinology, Baqai Medical University, Karachi, Pakistan.

Abstract

Objective: To determine the frequency of urinary tract infections and antibiotic sensitivity among patients with diabetes.

Methods: This observational study was carried out in Microbiology Department of Baqai Institute of Diabetology and Endocrinology (BIDE), Baqai Medical University from April 2015 to June 2016. All patients with diabetes having symptoms of UTI attending out patients department of BIDE were analyzed. All samples received in the laboratory were processed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Antimicrobial susceptibility pattern was determined by disc diffusion method.

Results: A total number of 199 urine specimens, frequency of UTI were 24 (12.06%) in male and 175 (87.94%) in female. UTIs were highly found in (age group 51-60) 70 (35.18%). Escherichia coli was the most frequent pathogen (71%), followed by Klebsiellapneumoniae (7.48%), Proteus mirabilis (1.87%), Staphylococcus aureus (9.35%), Candida (5.61%) and Candidaalbicans were (2.80%). Majority of gram negative uropathogens were shown high sensitivity towards Imipenem and Piperacillin / Tazobactam followed by Nitrofurantion, Ceftriaxone, Levofloxacin, Ofloxacine, Ciprofloxacin, Norfloxacin, Cefixime, Nalidixic acid and Cephradine. Gram positive was most sensitive to Nitrofurantionand Vancomycin followed by Piperacillin / Tazobactam, Imipenem, Cephradine, Ceftriaxone, Norfloxacin and Cefixime.

Conclusion: We observed the higher frequency of UTIs in female as compared to male participants due to poor hygiene. E.coli was the most frequent pathogen responsible for UTI in patients with diabetes, followed by Staphylococcus aureus.

Keywords: Antibiotics sensitivity; Diabetes Mellitus; Frequency of UTIs; Uropathogens.