Quantitation of Inositol Phosphates by HPLC-ESI-MS

Quynh H Duong; Ronald B Pegg

doi:10.1007/978-1-0716-0167-9_2

Quantitation of Inositol Phosphates by HPLC-ESI-MS

Methods Mol Biol. 2020:2091:31-37. doi: 10.1007/978-1-0716-0167-9_2.

Authors

Quynh H Duong¹, Ronald B Pegg²

Affiliations

¹ Department of Food Science and Technology, College of Agricultural and Environmental Sciences, The University of Georgia, Athens, GA, USA.
² Department of Food Science and Technology, College of Agricultural and Environmental Sciences, The University of Georgia, Athens, GA, USA. rpegg@uga.edu.

PMID: 31773567
DOI: 10.1007/978-1-0716-0167-9_2

Abstract

The coupling of anion exchange high-pressure liquid chromatography (HPLC) with electrospray ionization mass spectrometry (ESI-MS) allows for the simultaneous detection of the six forms of inositol phosphate (InsP). Here we describe a rapid quantitative analysis of InsPs by HPLC-ESI-MS, which can be applied to a wide array of sample types. With this method, InsPs could be separated and detected within 20 min of sample injection. The detection limit was as low as 25 pmol (i.e., ca. 2 nmol/g sample) for each type of InsP, which is particularly important for analytes that are often present at low abundance in nature.

Keywords: HPLC; HPLC-ESI-MS; Inositol phosphate; Ion-exchange HPLC; Phytic acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Inositol Phosphates / analysis*
Limit of Detection
Plants / chemistry*
Spectrometry, Mass, Electrospray Ionization
Stereoisomerism

Substances

Inositol Phosphates