Establishment and characterization of novel human primary endometrial cancer cell line (ZJB-ENC1) and its genomic characteristic

Xiaozhen Liu; Zhuozhuo Ren; Yu Xu; Wei Sun; Yongfeng Li; Xinmiao Rui; Dafei Xie; Xuli Meng; Zhiguo Zheng

doi:10.7150/jca.33013

Establishment and characterization of novel human primary endometrial cancer cell line (ZJB-ENC1) and its genomic characteristic

J Cancer. 2019 Oct 20;10(25):6466-6474. doi: 10.7150/jca.33013. eCollection 2019.

Authors

Xiaozhen Liu^{1

2

3}, Zhuozhuo Ren⁴, Yu Xu⁵, Wei Sun^{1

2

3}, Yongfeng Li^{1

6

7}, Xinmiao Rui⁸, Dafei Xie⁹, Xuli Meng¹⁰, Zhiguo Zheng^{1

2

3}

Affiliations

¹ Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, Hangzhou, 310022, China.
² The Experimental Center, Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, 310022, China.
³ The Experimental Center, Zhejiang cancer hospital , Hangzhou, 310022, China.
⁴ Medical Support Department, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310022, China.
⁵ Department of Hematology, The First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, 310022, China.
⁶ Department of Breast Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Hangzhou, 310022, China.
⁷ Department of Breast Surgery, Zhejiang cancer hospital, Hangzhou, 310022, China.
⁸ The Second Clinical Department, Zhejiang Chinese Medical University, Hangzhou, 310022, China.
⁹ General Surgery Department, Zhejiang Hospital, Hangzhou, 310022, China.
¹⁰ Department of Breast Thyroid Surgery, Tongde Hospital of Zhejiang Province, Hangzhou, 310022, China.

Abstract

The establishment of human malignant tumor cell lines can provide abundant experimental materials for understanding the biological characteristics of tumors, studying the carcinogenesis, molecular genetics and the mechanism of metastasis and evolution. In this study, a novel cell line designated ZJB-ENC1 has been established from poorly differentiated endometrioid adenocarcinoma. Cytological results showed monolayer-cultured cells were polygonal in shape and a piling-up tendency without contact inhabitation. Immunohistochemistry analysis showed that the cells were negative for ER, PR, c-erbB2, E-CAD, CD117, and OCT3/4, but strongly positive for PTEN and P16. Meanwhile, the tumorigenicity of ZJB-ENC1 was confirmed by subcutaneous transplantation of the cells into a xenograft mouse model. In addition, the results of the whole exome sequencing revealed a unique genomic characteristic of ZJB-ENC1 cells, all common and novel SNPs and InDels were identified. In conclusion, this new stable cell line may promote basic and clinical research on endometrial cancer (EC).

Keywords: Cell line; Endometrioid adenocarcinoma; Immunohistochemisty; Tumorigenicity; Whole exome sequencing.