In this study, a novel detection method of methimazole was proposed based on the hollow molecularly imprinted quartz crystal microbalance (QCM) sensor, in which the hollow imprinted polymers (H-MIPs) were firstly prepared through the surface imprinted techniques, using hollow silica spheres as matrix supporting material and methimazole as template molecule. The characterizations of H-MIPs were carefully studied. Compared with traditional MIPs, H-MIPs exhibited faster mass transfer rate and higher adsorption capacity. After coating onto the surface of Au chip, the H-MIPs QCM sensor was fabricated. Based on the frequency shift, good linear behavior in the range of 5-70 μg L-1, limit of detection of 3 μg L-1, and good recoveries of 88.32%-107.96% in the spiked pork, beef and milk were obtained. The analysis process could complete within 8 min. The developed sensor provided an effective, fast and accurate method for the methimazole detection in food samples.
Keywords: Azobisisobutyronitrile (PubChem CID: 6547); Ethylene glycol dimethacrylate (PubChem CID:7355); Food samples; Hollow molecularly imprinted polymer; Methacrylic acid (PubChem CID: 4093); Methimazole; Methimazole (PubChem CID: 1349907); N-isopropylacrylamide (PubChem CID: 16637); Quartz crystal microbalance; Sensor; Tetraethyl orthosilicate (PubChem CID: 6517); acetic acid (PubChem CID: 176).
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