Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect

C M López-Domínguez; M O Ramírez-Sucre; I M Rodríguez-Buenfil

doi:10.1016/j.btre.2019.e00384

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect

Biotechnol Rep (Amst). 2019 Oct 25:24:e00384. doi: 10.1016/j.btre.2019.e00384. eCollection 2019 Dec.

Authors

C M López-Domínguez¹, M O Ramírez-Sucre¹, I M Rodríguez-Buenfil¹

Affiliation

¹ Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Unidad Sureste, Interior del Parque Científico y Tecnológico Yucatán, Tablaje catastral No. 31264, km 5.5 carretera Sierra Papacal-Chuburná Puerto, 97302, Mérida, Yucatán, Mexico.

Abstract

In the present work the effect of three factors: pH, temperature and type of microorganism using a factorial design 3²x2 was evaluated on: growth, total cellulases hydrolytic activity (FPases), endoglucanases hydrolytic activity (CMCases), free reducing sugars (FRS), glucose, sucrose, and alcohol production using a culture medium based on mineral salts added with cladodes flour of Opuntia ficus-indica at 20% as the unique carbon source. Two wild microorganisms were used, Acinetobacter pittii a bacteria isolated from decaying cladodes, and Kluyveromyces marxianus a yeast isolated from termite stomach. The maximum hydrolytic activities were obtained with Acinetobacter pittii at 37 °C and pH 6.5 for total cellulases (0.67 ± 0.02 IU/ml) and for endoglucanases (0.23 ± 0.02 IU/ml) at 24 and 4 h. The maximum production of alcohol was 12.98 ± 0.06 g/L obtained with Kluyveromyces marxianus at 4 h with conditions of 40 °C and pH 5.5.

Keywords: Acinetobacter pittii; CF, Cladode flour; CFM, Cladode flour medium; CMC, Carboxymethylcellulose; CMCase, activity Carboximethylcellulase activity; FPase, activity Filter paperase activity; IU, International unit; Kluyveromyces marxianus; Nopal cladodes; Opuntia ficus-indica.