Polymerization-driven successive collapse of DNA dominoes enabling highly sensitive cancer gene diagnosis

Chem Commun (Camb). 2019 Dec 5;55(98):14797-14800. doi: 10.1039/c9cc07508a.

Abstract

We propose a novel fluorescence assay method by designing a polymerization-driven DNA dominoes collapse (PDDC) strategy, enabling highly sensitive detection of p53 gene (as a model analyte) and single nucleotide polymorphism analysis.

MeSH terms

  • Cell Line, Tumor
  • DNA Probes / chemistry*
  • DNA Probes / metabolism
  • DNA, Neoplasm / analysis*
  • DNA, Neoplasm / metabolism
  • Fluorescence Resonance Energy Transfer
  • Humans
  • Inverted Repeat Sequences
  • Neoplasms / diagnosis
  • Neoplasms / genetics
  • Nucleic Acid Hybridization
  • Polymerization
  • Polymorphism, Single Nucleotide
  • Tumor Suppressor Protein p53 / genetics*
  • p-Dimethylaminoazobenzene / analogs & derivatives
  • p-Dimethylaminoazobenzene / chemistry

Substances

  • DNA Probes
  • DNA, Neoplasm
  • TP53 protein, human
  • Tumor Suppressor Protein p53
  • 4-(4-dimethylaminophenylazo)benzoic acid
  • p-Dimethylaminoazobenzene