Identification of Reg3β-producing cells using IL-22-stimulated enteroids

Mika Sato; Akihiko Inaba; Ken Iwatsuki; Yuki Saito; Miki Tadaishi; Makoto Shimizu; Kazuo Kobayashi-Hattori

doi:10.1080/09168451.2019.1695575

Identification of Reg3β-producing cells using IL-22-stimulated enteroids

Biosci Biotechnol Biochem. 2020 Mar;84(3):594-597. doi: 10.1080/09168451.2019.1695575. Epub 2019 Nov 23.

Authors

Mika Sato¹, Akihiko Inaba², Ken Iwatsuki², Yuki Saito¹, Miki Tadaishi¹, Makoto Shimizu¹, Kazuo Kobayashi-Hattori¹

Affiliations

¹ Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
² Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

PMID: 31760857
DOI: 10.1080/09168451.2019.1695575

Abstract

Reg3β, a lectin, displays antibacterial activity. This study investigated Reg3β-expressing cells using IL-22-stimulated enteroids. IL-22 stimulation elevated the mRNA and protein levels of Reg3β. IL-22 also increased the mRNA levels of CD133 (a transit-amplifying cell marker) and lysozyme (a Paneth cell marker). Immunohistochemistry showed partial colocalization of Reg3β- and lysozyme-positive cells, suggesting that Paneth cells are one of Reg3β-producing cells.

Keywords: Enteroids; IL-22; Paneth cells; Reg3β.

MeSH terms

Animals
Biomarkers / metabolism
Interleukin-22
Interleukins / pharmacology
Lectins / biosynthesis*
Lectins / genetics
Lectins / metabolism
Paneth Cells / drug effects*
Paneth Cells / metabolism

Substances

Biomarkers
Interleukins
Lectins