Optimization of the radiolabelling method for improved in vitro and in vivo stability of 90Y-albumin microspheres

Vukadinović Aleksandar; Janković Drina; Radović Magdalena; Milanović Zorana; Mirković Marija; Stanković Dragana; Vranješ-Đurić Sanja

doi:10.1016/j.apradiso.2019.108984

Optimization of the radiolabelling method for improved in vitro and in vivo stability of ⁹⁰Y-albumin microspheres

Appl Radiat Isot. 2020 Feb:156:108984. doi: 10.1016/j.apradiso.2019.108984. Epub 2019 Nov 12.

Authors

Vukadinović Aleksandar¹, Janković Drina¹, Radović Magdalena¹, Milanović Zorana¹, Mirković Marija¹, Stanković Dragana¹, Vranješ-Đurić Sanja²

Affiliations

¹ University of Belgrade, Vinča Institute of Nuclear Sciences, P.O.Box 522, Belgrade, 11000, Serbia.
² University of Belgrade, Vinča Institute of Nuclear Sciences, P.O.Box 522, Belgrade, 11000, Serbia. Electronic address: sanjav@vinca.rs.

PMID: 31760344
DOI: 10.1016/j.apradiso.2019.108984

Abstract

Biologically stable ⁹⁰Y-labelled albumin microspheres (AMS) were developed by optimizing the process of their preparation. Three formulations of ⁹⁰Y-AMS were initially prepared with high radiolabelling yield but depending on the step when the radionuclide ⁹⁰Y and DTPA chelator were added, radiolabelled microspheres with different in vitro and in vivo stability were obtained. DTPA was proved as a useful chelating agent that tightly links radionuclide ⁹⁰Y to albumin. Also, AMS radiolabelled via DTPA during preparation and before microspheres stabilization, showed significant in vitro and in vivo stability ready for the potential use in selective internal radiation therapy.

Keywords: (90)Y; DTPA; Human albumin microspheres; In vivo stability; Radiolabelling.

MeSH terms

Albumins / chemistry*
Animals
Chelating Agents / chemistry
Male
Microspheres*
Pentetic Acid / chemistry
Radiopharmaceuticals / chemistry*
Rats
Rats, Wistar
Yttrium Radioisotopes / chemistry*

Substances

Albumins
Chelating Agents
Radiopharmaceuticals
Yttrium Radioisotopes
Pentetic Acid