Polyhydroxylated sterols from Monascus purpureus-fermented rice

Steroids. 2020 Feb:154:108546. doi: 10.1016/j.steroids.2019.108546. Epub 2019 Nov 21.

Abstract

Using a cell-based cytotoxicity assay, two new polyhydroxylated sterols, 16(S),22(S)-epoxy-3β,5α,6β,20(R),23(R),25-hexahydroxy-7-ergostene and 3β,7β,8α,25-tetrahydroxy-5,22E-ergostadiene were isolated from the ethyl acetate portion of the ethanolic extract of Monascus purpureus. Their structures were elucidated by spectroscopic analysis and in comparison with those reported in the literature. Both compounds showed cytotoxic activity against the lung adenocarcinoma (A549) with IC50 values of 12.6 and 18.5 μM, exhibited moderate activities against human ovarian cancer (A2780), with IC50 values of 8.8 and 9.4 μM.

Keywords: Cytotoxicity; Ergosterol derivatives; Monascus purpureus; Steroids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A549 Cells
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Fermentation*
  • Humans
  • Hydroxylation
  • Molecular Conformation
  • Monascus / chemistry*
  • Monascus / metabolism
  • Oryza / metabolism*
  • Sterols / chemistry
  • Sterols / isolation & purification
  • Sterols / pharmacology*
  • Structure-Activity Relationship
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • Sterols